Thermostable Variants of Cocaine Esterase for Long-Time Protection against Cocaine Toxicity
نویسندگان
چکیده
منابع مشابه
Thermostable variants of cocaine esterase for long-time protection against cocaine toxicity.
Enhancing cocaine metabolism by administration of cocaine esterase (CocE) has been recognized as a promising treatment strategy for cocaine overdose and addiction, because CocE is the most efficient native enzyme for metabolizing the naturally occurring cocaine yet identified. A major obstacle to the clinical application of CocE is the thermoinstability of native CocE with a half-life of only a...
متن کاملRapid and robust protection against cocaine-induced lethality in rats by the bacterial cocaine esterase.
There is no approved means to prevent the toxic actions of cocaine. Cocaine esterase (CocE) is found in a rhodococcal strain of bacteria that grows in the rhizosphere soil around the coca plant and has been found to hydrolyze cocaine in vitro. The esteratic activity of CocE (0.1-1.0 mg, i.v.) was characterized and confirmed in vivo by assessing its ability to prevent cocaine-induced convulsions...
متن کاملCocaine esterase prevents cocaine-induced toxicity and the ongoing intravenous self-administration of cocaine in rats.
Cocaine esterase (CocE) is a naturally occurring bacterial enzyme, is a very efficient protein catalyst for the hydrolysis of cocaine, and has previously been shown to protect rodents from the lethal effects of cocaine. The current studies were aimed at evaluating the capacity of a longer acting mutant form (CocE T172R/G173Q; DM CocE) of CocE to protect against the lethal effects of cocaine, an...
متن کاملSilent Cocaine Poisoning
Background: Cocaine poisoning is known for causing severe clinical effects such as tachycardia, hypertension, agitation and confusion. Absence of clinical manifestations in cocaine poisoning is unusual. Case report: A 26-year old man, known to be a cocaine addict, declared that he was forced by cocaine dealers to swallow many tablets of cocaine, six hours prior to admission to emergency departm...
متن کاملMechanisms of acute cocaine toxicity.
Patients with acute cocaine poisoning present with life-threatening symptoms involving several organ systems. While the effects of cocaine are myriad, they are the result of a limited number of cocaine-protein interactions, including monoamine transporters, neurotransmitter receptors and voltage-gated ion channels. These primary interactions trigger a cascade of events that ultimately produce t...
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ژورنال
عنوان ژورنال: Molecular Pharmacology
سال: 2008
ISSN: 0026-895X,1521-0111
DOI: 10.1124/mol.108.049486